Production in Escherichia coli of extracellular Serratia spp. hydrolases
AssigneeBenzon Pharma A/S
InventorsSoren Molin, Michael Givskov, Erik Riise
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Abstract
Extracellular Serratia spp. enzymes have been found to be excreted by another gram-negative organism harbouring a plasmid carrying DNA from Serratia spp. encoding the enzymes. This organism, e.g. E. coli, is therefore employed to produce the enzymes, specific examples of Serratia spp. enzymes produced are a nuclease, a lipase and a phosopholipase. The nuclease may be employed to remove nucleic acids from a biological material.
Claims10
A method of producing an extracellular hydrolytic Serratia spp. enzyme selected from the group consisting of exocellular nuclease, exocellular phospholipase and exocellular lipase, comprising cultivating, E. coli in a culture medium, said E. coli harboring a hybrid plasmid which comprises DNA encoding an extracellular hydrolytic Serratia spp. enzyme selected from the group consisting of exocellular nuclease, exocellular phospholipase and exocellular lipase, operably linked to a promotor functional in said E. coli, under conditions conductive to the expression of said enzyme and its secretion into the culture medium, and harvesting the enzyme from the culture medium.
A DNA fragment comprising a DNA sequence encoding a Serratia spp. phospholipase.
Dependencies are inferred from the claim text.