US 5,173,417 A · Thermostable lipoprotein lipase, process for producing the same, and triglyceride determining reagent using the same

Abstract

The present invention is directed toward a thermostable lipoprotein lipase capable of hydrolyzing triglycerides in lipoproteins to glycerol and fatty acids, a process for producing the thermostable lipoprotein lipase, and a blood triglyceride determining reagent containing the thermostable lipoprotein lipase. The thermostable lipoprotein lipase exhibits about 100% retention of the hydrolyzing activity when treated in a buffer having a pH of from about 4 to 7 at about 60.degree. C. for about 15 minutes and a glycerol forming activity/fatty acid forming activity ratio of at least about 15%. The process comprises cultivating a thermophilic actinomycetes, particularly Streptomyces 7825 (FERM P-9983, FERM BP-2489 and recovering lipoprotein lipase from the culture.

Claims3

Dependent claims

A thermostable lipoprotein lipase capable of hydrolyzing triglycerides in lipoproteins to glycerol and fatty acids, said hydrolyzing activity being about 100% retained after treatment in a buffer having a pH of from about 4 to 7 at about 60.degree. C. for about 15 minutes,

wherein said thermostable lipoprotein lipase has a glycerol forming activity and a fatty acid forming activity, such that said glycerol forming activity is at least about 15% of said fatty acid forming activity;
has a pH optimum of from about 8 to 9;
has a stable pH of from about 4 to 7;
and a temperature optimum of about 50.degree. C. to 60.degree. C.

1 more element

Dependencies are inferred from the claim text.

Thermostable lipoprotein lipase, process for producing the same, and triglyceride determining reagent using the same

Life of this patent

Thermostable lipoprotein lipase, process for producing the same, and triglyceride determining reagent using the same

Life of this patent

Abstract

Claims3