Automated DNA sequencing technique
AssigneeCalifornia Institute of Technology
InventorsLloyd M. Smith, Leroy E. Hood, Michael W. Hunkapiller, Tim J. Hunkapiller
Life of this patent
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Abstract
A system for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations comprising: a source of chromophore or fluorescent tagged DNA fragments; a zone for contacting an electrophoresis gel; means for introducing said tagged DNA fragments to said zone; and photometric means for monitoring said tagged DNA fragments as they move through said gel.
Claims7
A novel system for the electrophoretic analysis of oligonucleoide fragments produced in sequencing operations comprising:
- a source of chromophore of fluorescent tagged oligonucleotide fragments, said chromophore or fluorophore being distinguishable by its spectral characteristics,
- a zone for containing an electrophoresis medium,
- means for introducing said tagged oligonucleotide fragments to said zone;
- and
- photometric means for monitoring and distinguishing said tagged DNA fragments upon separation in said medium.
A novel system for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations by the chain termination method, comprising:
- a source of chromophore or fluorescent tagged DNA fragments from sequencing operations wherein the primer oligonucleotide from each of the four sequencing reactions A, C, G, and T has a different chromophore or fluorescent tag attached to it, each tag being distinguishable from the others by its spectral characteristics.
- a zone containing an electrophoresis medium;
- means for introducing said tagged DNA fragments to said zone;
- and
- photometric means for monitoring and distinguishing said tagged DNA fragments upon separation in said medium.
Dependencies are inferred from the claim text.