Immobilized polyethylene oxide star molecules for bioapplications
AssigneeMassachusetts Institute of Technology
InventorEdward W. Merrill
Life of this patent
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Abstract
This invention pertains to a method for immobilizing polyethylene oxide (PEO) star molecules in the form of hydrogels. the PEO star molecules are biocompatible and demonstrate non-thrombogenic properties. As such, the PEO star molecules have numerous uses for biomedical applications. The hydrogels contain a high percentage of terminal hydroxyl groups for attachment of affinity ligands and can be used for separating and purifying therapeutic proteins.
Claims16
A method of immobilizing polyethylene oxide star molecules to a support surface in the form of a hydrogel, comprising the steps of:
- a) providing a solution comprising polyethylene oxide star molecules consisting essentially of a plurality of hydroxy-terminated polyethylene oxide chains attached to a polymeric core, the star molecules being essentially non-adsorptive toward biomolecules and nonthrombogenic;
- b) depositing the solution onto a support surface;
- and
- c) reacting the hydroxy-terminated polyethylene oxide chains of the star molecules to thereby produce a hydrogel which is immobilized to the support surface.
A method of immobilizing polyethylene oxide star molecules to a support surface in the form of a hydrogel, comprising the steps of:
- a) providing an aqueous solution comprising polyethylene oxide star molecules consisting essentially of a plurality of hydroxy-terminated polyethylene oxide chains attached to a divinyl benzene core, the star molecules being essentially non-adsorptive toward bimolecules and nonthrombogenic;
- b) depositing the aqueous solution onto a support surface;
- and
- c) irradiating the solution with electron radiation to sufficiently crosslink the polyethylene oxide chains to produce a hydrogel of crosslinked star molecules which is thereby immobilized to the support surface.
A method of immobilizing polyethylene oxide star molecules to a support surface in the form of a hydrogel, comprising the steps of:
- a) exposing an organic solution comprising polyethylene oxide star molecules consisting essentially of a plurality of hydroxy-terminated polyethylene oxide chains attached to a divinyl benzene core to tresyl chloride to affix tresyl groups to the hydroxy termini;
- b) transferring the tresylated polyethylene oxide star molecules from the organic solvent to an aqueous solution;
- c) adjusting the pH of the aqueous solution to about 10 or above;
- and
- d) contacting the solution of step (c) with a support surface containing amino and/or thiol groups to covalently bind the tresylated star molecules, thereby immobilizing the tresylated star molecules in a dense layer to the support surface.
Dependencies are inferred from the claim text.