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US 5,166,813 AGranted
Optical evaluation using a hologram barrier filter
AssigneeNygene Corporation
InventorMichael H. Metz
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AssigneeNygene Corporation
InventorMichael H. Metz
Estimating the term…
Optical detection apparatus and process which, in its optimum configuration, utilizes holographic optical elements both as a dichroic beamsplitter and as a selective notch-type barrier filter to enhance the amount of light to reach the detector while barring undesirable wavelengths from reaching the detector. The apparatus, when incorporated into a fluorescent-light detecting process, has the advantage of minimizing loss of light suffered because of a small Stokes shift, which shift occurs between the wavelength of the light transmitted to a sample target area and the increased wavelength of fluorescent light emitted from the sample for collection by a detection device. This advantage which is allowed by sharper cut-on of optical transmission properties relative to wavelength by a barrier filter (or filters) comprised of holographic optical element (or elements) provides an important increase in the fluorescence-collecting capacity of the apparatus and the sensitivity of detection processes carried out with the apparatus. Those optical detection systems which use a dichroic beamsplitter benefit by using a holographic optical element which can serve multiple purposes by acting as an exciter filter, selecting the wavelength band from the input light, as a highly efficient mirror to enhance the amount of light reaching the sample and, also, as a barrier filter as described above.
An optical detection apparatus for use in measuring fluorescence emitted from a sample in a target area having a beamsplitter for directing light within a first wavelength or wavelength band from a light source to the target area to stimulate a sample, wherein the light emitted from the sample contains the first wavelength or wavelength band and a second wavelength or wavelength band characteristic of the sample;
An optical detection apparatus for use in measuring fluorescence emitted from a sample in a target area having a beamsplitter for directing light within a first wavelength or wavelength band from a light source to the target area to stimulate a sample, wherein light emitted from the target area or sample contains the first wavelength or wavelength band and a second wavelength or wavelength band characteristic of the sample;
An optical detection apparatus for use in measuring fluorescence emitted from a sample in a target area having an exciter filter for limiting the wavelength or wavelength band emitted from a light source to a first wavelength or wavelength band to be incident upon a target area to stimulate a sample, a beamsplitter for directing light within said first wavelength or wavelength band from a light source to a target area to stimulate said sample, wherein light emitted from the sample contains all or part of the first wavelength or wavelength band and a second wavelength or wavelength band characteristic of the sample;
An optical detection apparatus for use in measuring fluorescence emitted from a sample in a target area having a barrier filter for limiting the wavelengths of light returned from a sample to a photodetector, said sample which has been stimulated by light from a light source, said light within a first wavelength or wavelength band and light emitted from said sample contains said first wavelength or wavelength band and a second wavelength or wavelength band;
An optical detection apparatus for use in measuring fluorescence emitted from a sample in a target area having an exciter filter for limiting the wavelength or wavelength band emitted from a light source to be incident upon a target area to stimulate a sample and a barrier filter for limiting the wavelengths of light to be returned from the target area or sample to a photodetector;
A method for measuring fluorescence emitted from a sample in a target area which includes:
A method for measuring fluorescence emitted from a sample in a target area wherein the light returned from said target area is comprised of one or more contiguous or non-contiguous wavelengths or wavelength bands of those used to stimulate said sample and one or more contiguous or non-contiguous additional wavelengths or wavelength bands characteristic of the sample;
Dependencies are inferred from the claim text.