Cysteine added variants of interleukin-3 and chemical modifications thereof
AssigneeGenetics Institute, Inc.
InventorsGray Shaw, Geertruida Veldman, Joseph L. Wooters
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Abstract
Cysteine added variants ("CAVs") of interleukin-3 are provided having one or more cysteine residues substituted for selected naturally occurring amino acid residues, or inserted into the polypeptide sequence, and preferably being further modified by deletion of certain N-terminal amino acids. Such CAVs may be additionally modified by the coupling of sulfhydryl reactive compounds to the introduced cysteine residue(s) without loss of bioactivity to produce selected homogeneously modified IL-3 and improved pharmaceutical compositions containing the same.
Claims9
A cysteine added variant of human interleukin-3 characterized by having an added cysteine residue in the N-terminal region between amino acids 1 through 14 of the natural human interleukin-3 sequence, said added cysteine residue either being substituted for an amino acid in the natural human interleukin-3 sequence or being inserted between two amino acids of the natural human interleukin-3 sequence, said cysteine added variant being further characterized by having a polyethylene glycol moiety covalently attached to said added cysteine residue.
Dependencies are inferred from the claim text.