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US 5,164,299 AGranted
Use of a mixture of conjugated and unconjugated solid phase binding reagent to enhance the performance of assays
AssigneeE. I. Du Pont de Nemours and Company
InventorStephen B. Lambert
Life of this patent
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Abstract
In a solid phase homogeneous or heterogeneous assay for detection or quantitation of an analyte in a biological fluid, use of a combination of unconjugated binding reagent and carrier-conjugated binding reagent immobilized on the solid phase provides enhanced assay performance.
Claims15
A solid phase heterogeneous or homogeneous immunoassay to detect or quantitate an analyte in a liquid sample which comprises:
- a) reacting binding reagent, immobilized on a solid phase, with sample;
- and
- b) determining the extent of binding which occurs in step (a);
- wherein the immobilized binding reagent is a mixture of carrier-conjugated and unconjugated binding reagent in which the ratio of carrier-conjugated binding reagent to unconjugated binding reagent is in the range from 1:19 to 19:1.
1 more element
Dependencies are inferred from the claim text.