Nucleoside oxidase and assay method utilizing same
AssigneeOtsuka Foods Co., Ltd.
InventorsYoshikazu Isono, Masami Hoshino
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Abstract
The present invention provides a novel nucleoside oxidase YT-1 serving as an enzyme for catalyzing oxidation reaction of a nucleoside, and oxidase being characterized in that the oxidase causes the nucleoside to react with molecular oxygen to produce a nucleoside-5'-carboxylic acid via a nucleoside-5'-aldehyde without producing hydrogen peroxide, a process for producing the enzyme, a novel microorganism having ability to produce the enzyme, and a novel analysis or assay method for nucleosides and the like utilizing the enzyme.
Claims7
An isolated and purified nucleoside oxidase YT-1 which is an enzyme for catalyzing an oxidation reaction of a nucleoside, wherein the oxidase causes the nucleoside to react with molecular oxygen to produce a nucleoside-5'-carboxylic acid via a nucleoside-5'aldehyde without producing hydrogen peroxide.
A process for producing a nucleoside oxidase YT-1 comprising:
- (1) incubating on a nutrient medium a microorganism which is a Pseudomonas maltophilia LB-86 and which is able to produce said nucleoside oxidase YT-1 which is an enzyme for catalyzing an oxidation reaction of a nucleoside, wherein the oxidase causes the nucleoside to react with molecular oxygen to produce a nucleoside-5'-carboxylic acid via a nucleoside-5'-aldehyde without producing hydrogen peroxide, and
- (2) collecting the oxidase YT-1 from the resulting culture.
A biologically pure culture of a microorganism, wherein the microorganism is a Pseudomonas maltophilia LB-86 and is able to produce a nucleoside oxidase YT-1 which is an enzyme for catalyzing an oxidation reaction of a nucleoside, wherein the oxidase causes the nucleoside to react with molecular oxygen to produce a nucleoside-5'-carboxylic acid via a nucleoside-5'-aldehyde without producing hydrogen peroxide.
A method of assaying a sample for the presence of a nucleoside, comprising
- (1) reacting:
- (a) a nucleoside oxidase YT-1 which is an enzyme for catalyzing an oxidation reaction of a nucleoside, wherein the oxidase causes the nucleoside to react with molecular oxygen to produce a nucleoside-5'-carboxylic acid via a nucleoside-5'-aldehyde without producing hydrogen peroxide, with
- (b) a mixture of the sample and a chromogenic reagent forming a color on oxidation, and
- (2) measuring the degree of color formation by the chromogenic reagent which is proportional to the variation in the quantity of nucleoside in the sample.
Dependencies are inferred from the claim text.