Oligosaccharide conjugate vaccines
AssigneeAmerican Cyanamid Company
InventorMassimo Porro
Life of this patent
Estimating the term…
Abstract
The present invention relates to an improved method for producing oligosaccharide conjugate vaccines. In an additional aspect of the invention, oligosaccharide vaccines are produced which elicit a monospecific and homogeneous immune response to capsular polysaccharide. A specific embodiment of the invention provides for vaccines which induce immunity to prevalent serotypes of Streptococcus pneumoniae.
Claims27
A method for producing a covalent conjugate of an oligosaccharide and a carrier protein, comprising the following steps:
- (i) reacting an oligosaccharide having a terminal reducing group with diaminomethane in the presence of pyridine borane such that reductive animation occurs;
- and
- (ii) reacting the animated oligosaccharide product of (i) with a molecular comprising two functional groups, one of which is capable of reacting with the terminal group of the activated oligosaccharide and the other which is capable of reacting with said carrier protein;
- and
- (iii) reacting the activated oligosaccharide product of (ii) with said carrier protein such that conjugation occurs.
A covalent conjugate between oligosaccharides and a carrier protein produced by a method comprising the steps of:
- (i) hydrolyzing a polysaccharide to produce oligosaccharides which have at least one terminal reducing group;
- and
- (ii) reacting said oligosaccharides with diaminomethane in the presence of pyridine borane such that reduce animation occurs;
- and
- (iii) reacting the animated oligosaccharide product of (i) with a molecule comprising two functional groups, one of which is capable of reacting with the terminal group of the activated oligosaccharide and the other which is capable of reacting with said carrier protein;
- and
- (iv) reacting the activated oligosaccharide product of (ii) with said carrier protein such that conjugation occurs.
Dependencies are inferred from the claim text.