Method and kit for determining the functional activity of protein S in human plasma
AssigneeInstrumentation Laboratory Company
InventorsLuigi Preda, Antonio Lombardi
Life of this patent
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Abstract
To determine the biological activity of protein S in a sample of human plasma, the suitably diluted sample is added to a substrate formed from plasma deficient in protein S, in which protein C is activated. The functionality of protein is evaluated by a coagulometric test using bovine thromboplastin with added calcium as the phospholipid source. Also disclosed is a kit for determining the activity of Protein S in a human plasma sample. The kit includes a first reagent which is human plasma deficient in protein S, a second reagent which contains an activator for protein C, and a third reagent which contains bovine thromboplastin.
Claims14
A method for determining the activity of protein S in a human plasma sample comprising combining the plasma sample with activated substrate plasma to form a mixture, then combining the mixture with bovine thromboplastin in the presence of calcium ions to initiate a coagulation reaction and measuring a parameter related to the time within which the coagulation reaction occurs.
A kit for determining the activity of protein S in a human plasma sample which comprises:
- a) a first reagent whcih is human plasma deficient in protein S,
- b) a second reagent which contains an activator for protein C, and
- c) a third reagent which contains bovine thromboplastin and calcium ions.
Dependencies are inferred from the claim text.