Media for normal human muscle satellite cells
AssigneeUniversity of Colorado Foundation, Inc.
InventorsRichard G. Ham, Judith A. St. Clair
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Abstract
An improved basal nutrient medium MCDB 120 for the clonal growth of human muscle satellite cells (HMSC) is described. In addition, a serum-free supplement for the clonal growth of HMSC containing serum albumin, dexamethasone, fetuin epidermal growth factor and insulin. Combination of MCDB 120 and the serum-free supplement results in a serum-free medium for growth of HMSC. The serum-free supplement can also be used with other basal nutrient media such as MCDB 131M for growth of HMSC. Addition of 5% dialyzed fetal bovine serum to the serum-free media described results in semi-defined media which allows significantly improved growth of HMSC.
Claims22
Human muscle satellite cell growth with serumfree supplement comprising nutrient medium MCDB120:
- about 0.5 mg/ml serum albumin;
- about 1 .times. 10.sup.-6 M dexamethasone;
- about 10 ng/ml epidermal growth factor;
- and about 0.5 mg/ml fetuin.
The medium according to claim 5 wherein the concentration of fibroblast growth factor is about 30 ng/ml.
The improved method of claim 9 wherein said medium further comprises about 1 .times. 10.sup.-5 M insulin.
A serum-free human muscle satellite cell growth medium supplement which comprises serum albumin, dexamethasone, epidermal growth factor and fetuin.
Dependencies are inferred from the claim text.