Process for the production of a polypeptide
OwnerIan B. Holland, Nigel Mackman +1inventors
InventorsIan B. Holland, Nigel Mackman, Jean-Marc Nicaud
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Abstract
A process for the production of a polypeptide in which host cells transformed with DNA coding for a fusion protein comprising the polypeptide and a further peptide comprising a C-terminal secretion sequence are cultured to express and secrete the fusion protein. The C-terminal secretion sequence is, for example, the haemolysin C-terminal secretion sequence.
Claims11
A DNA sequence coding for a fusion protein comprising a first polypeptide and a further peptide in which the further peptide comprises a C-terminal secretion sequence.
An expression vector containing a first DNA sequence coding for a peptide comprising a haemolysin C-terminal secretion sequence and a unique restriction site positioned relative thereto in such a way that expression of a fusion protein comprising the peptide and a further peptide may be obtained when a second DNA sequence coding for the polypeptide is inserted at the unique restriction site.
A process for the production of a polypeptide, in which host cells, which express transport proteins effective to secrete a peptide comprising a C-terminal secretion sequence, transformed or transfected with DNA coding for a fusion protein comprising the polypeptide and a further peptide comprising a C-terminal secretion sequence are cultured to express and secrete the fusion protein.
Dependencies are inferred from the claim text.