Transformed yeasts having steroid-hydroxylase activity and process for hydroxylation of steroids using the said transformed yeasts
AssigneeDirector-General of Agency of Industrial Science & Technology an organ of The Ministry of Industrial Trade and Industry of Japan
InventorsYoshiyasu Yabusaki, Toshiyuki Sakaki, Megumi Shibata, Hideo Ohkawa
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Abstract
Transformed yeasts with yeast expression plasmid containing yeast alcohol dehydrogenase promoter and bovine adrenocortical cytochrome P-450.sub.17.alpha. cDNA, which produce bovine adrenocortical cytochrome P-450.sub.17.alpha., and processes for producing 17-hydroxypregnenolone and 17-hydroxyprogesterone which comprises 17-hydroxylating pregnenolone or progesterone to 17-hydroxypregnenolone or 17-hydroxyprogesterone, respectively, with the said transformed yeasts.
Claims10
A yeast expression plasmid which comprises yeast alcohol dehydrogenase promoter and bovine adrenocortical cytochrome P-450.sub.17.alpha. cDNA, wherein the promoter is operably-linked to the cDNA.
An expression plasmid pA.alpha.1.
An expression plasmid pA.alpha.2.
Yeasts which are transformed with yeast expression plasmids which plasmids comprise yeast alcohol dehydrogenase promoter and bovine adrenocortical cytochrome P-450.sub.17.alpha. cDNA, wherein the promoter is operably-linked to the cDNA, and which plasmids produce bovine adrenocortical cytochrome P-450.sub.17.alpha..
A yeast harboring an expression plasmid selected from plasmids pA.alpha.1 or pA.alpha.2.
Saccharomyces cerevisiae AH22(pA.alpha.1).
Saccharomyces cerevisiae AH22(pA.alpha.2).
A process for producing bovine adrenocortical cytochrome P-450.sub.17.alpha., which comprises culturing yeasts which are transformed with yeast expression plasmids which plasmids comprise yeast alcohol dehydrogenase promoter and bovine adrenocortical cytochrome P-450.sub.17.alpha. cDNA, wherein the promoter is operably-linked to the cDNA, and which plasmids are capable of producing produce bovine adrenocortical cytochrome P-450.sub.17.alpha. in a suitable medium.
Dependencies are inferred from the claim text.