Apparatus and method for electroporation
AssigneeAmoco Corporation
InventorsHeinz H. Busta, Michael L. Bittner, Richard E. Cuellar
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Abstract
An electrode set for electrotransformation of a host cell by electroporation, comprising: an electrically insulating substrate; and at least one pair of interdigitated electrodes which are carried by said substrate and which lie in a generally planar array, said electrodes and said substrate being adopted to carry a cell transformant and a host cell thereon, said electrodes and said host cell and said cell transformant being adapted to pass electrical current upon the application of a potential difference across said electrodes.
Claims15
An apparatus for transforming of a host cell by electroporation, comprising:
- at least one electrically insulating substrate;
- and at least two electrodes which are carried by said substrate and which lie adjacent to each other, said electrodes and said substrate being adapted to be placed in close proximity to at least one cell transformant and at least one host cell, said electrodes and said host cell and said cell transformant being adapted to pass an electrical current therethrough upon the application of a potential difference across said electrodes, said electrodes having at least one of a difference in elevation relative to said substrate, projecting means for physically penetrating at least a portion of a surface of an organism carrying said host cells, and a difference in electrical conductivity.
Dependencies are inferred from the claim text.