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US 5,137,812 AGranted
Colony blotting method and device
AssigneeMinnesota Mining and Manufacturing Company
InventorRichard R. Matner
Life of this patent
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Abstract
A method and article for the identification of colonies of microbes involves the use of a reconstitutable dry culture medium plate in combination with a transfer membrane. Microbe-specific antigen can be extracted from the surface of the growth medium of the plate and its presence determined, e.g., by immunoassay.
Claims11
A method for identifying the presence of colonies of microbes, the method comprising the steps of:
- (1) inoculating a reconstitutable dry culture medium plate comprising (a) a substantially flat self-supporting body member and (b) a cover sheet adhered to at least a portion of the body member, with an inoculum from a sample suspected of containing microbes,
- (2) incubating the inoculated plate under conditions suitable to allow the growth of colonies of the microbes,
- (3) removing the cover sheet to expose a surface of the growth medium of the plate and contacting the exposed surface with a suitable transfer membrane, the transfer membrane provided separate from the cover sheet, in a manner that allows the membrane to extract microbe-specific antigen from the area of the microbial colonies,
- (4) removing the transfer membrane from the surface of the medium, and
- (5) determining the presence of microbe-specific antigen on the transfer membrane by suitable means.
2 more elements
A method for identifying the presence of colonies of microbes, the method comprising the steps of:
- (1) inoculating a reconstitutable dry culture medium plate comprising (a) a self-supporting body member, and (b) a cover sheet adhered to at least a portion of the body member, with an inoculum from a sample suspected of containing microbes, wherein the cover sheet is itself suitable, and used, as a transfer membrane,
- (2) incubating the inoculated plate under conditions suitable to allow the growth of colonies of the microbes,
- (3) removing the cover sheet from the surface of the growth medium of the plate in a manner that allows the cover sheet to serve as a transfer membrane to extract microbe-specific antigen from the area of the microbial colonies,
- (4) removing the transfer membrane from the surface of the medium, and
- (5) determining the presence of microbe-specific antigen on the transfer membrane by suitable means.
2 more elements
Dependencies are inferred from the claim text.