Method to identify analyte-bending ligands
AssigneeTerrapin Technologies, Inc.
InventorLawrence M. Kauvar
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Abstract
The invention provides methods to obtain specific paralogs which are capable of specifically binding a selected moiety. Such paralogs are useful for chromatographic separations and purifications of desired analytes and in binding assays such as immunoassays involving said analytes, as well as for any purpose which requires said specific binding. The invention also provides kits for these determinations, and methods to synthesize panels of candidate paralogs.
Claims30
A method to identify a paralog useful for the conduct of affinity chromatography with respect to an analyte which has specific affinity for a first moiety in comparison to additional moieties present in the environment of the first moiety which method comprises:
- screening, for ability to selectively bind said first moiety a panel of individual candidate paralogs, wherein said candidate paralogs have systematically varied values of at least two different parameters, each of which parameters determines the ability of the paralog to bind other substances.
A method to identify a paralog useful for the conduct of affinity chromatography with respect to an analyte in a sample containing additional components,
- wherein said paralog has specific affinity for said analyte in comparison to said additional components, which method comprises:
- screening, for ability to selectively bind said analyte, a panel of individual candidate paralogs, wherein said candidate paralogs have systematically varied values of at least two parameters, each of which parameters determines the ability of the paralog to bind other substances.
Dependencies are inferred from the claim text.