US 5,132,538 AGranted

Measuring percentage of protein in whole grain samples

AssigneeNIRSystems Incorporated

InventorKarl Norris

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Abstract

In a method for measuring protein content of whole grain kernels with a near infrared spectrophotometer, reflectance measurements are made at wavelengths shorter than 1600 nanometers. The wavelengths are selected to correspond with absorbance bands of the constituents of the sample. The measurements are encoded into absorbance data and are normalized by subtracting the absorbance value at a first wavelength and then dividing the resulting data by the value at a second wavelength. In accordance with one embodiment, the normalized data is substituted in a formula having coefficients determined by regression from samples having known percentages of protein in the sample to yield the percentage of protein in the unknown sample. In accordance with a second embodiment, the second differential of the absorbance data is determined and the resulting differential values are inserted in the formula having coefficients obtained from the known samples to yield the percentage or protein in the unknown sample.

Claims11

Dependent claims

A method of determining the percentage of protein in a whole grain sample, comprising determining a first set of values varying with the absorbance of said sample at a multiplicity of near infrared wavelengths, a plurality of said wavelengths being less than 1600 nanometers, normalizing said first set of values by first subtracting from each of said first set of values one of said first set of values to determine a second set of values, and then dividing each of said second set of values by one of the values of said second set to determine a set of normalized values, and determining the percentage of protein in said sample from the formula:

K.sub.0 +K.sub.1 f(R.sub.1)+K.sub.2 f(R.sub.2)+. . . K.sub.n f(R.sub.n), wherein f(R.sub.1) through f(R.sub.n) are values related to said set of normalized values, at least one of said values of f(R.sub.1) through f(R.sub.n) being related to one of said normalized values at a wavelength less than 1600 nanometers in an absorbance band of protein in said sample, and wherein K.sub.0 through K.sub.n are coefficients determined by multiple regression from a multiplicity of samples of grain, each of said multiplicity of samples containing a known amount of protein.

Dependencies are inferred from the claim text.