Back to results
US 5,132,439 AGranted
Protein staining compositions and methods
AssigneePromega Corporation
InventorsJohn W. Schultz, David L. Leland
Life of this patent
Estimating the term…
Abstract
The present invention provides novel dyes for staining protein, particularly those in elecrophoretic gels. The dyes of the invention are derivatives of Coomassie.RTM. dyes. The invention also provides quantitative and qualitative methods for detecting proteins which comprise staining the proteins with a dye of the invention.
Claims12
A dye of Formula X:
- ##STR3## or a salt thereof, wherein R.sup.10, R.sup.20, and R.sup.30 are independently selected from the group consisting of hydrogen and alkyl of 1-6 carbon atoms;
- R.sup.40 and R.sup.50 are independently selected from the group consisting of alkyl of 1-6 carbon atoms;
- R.sup.60 is selected from the group consisting of alkyl of 1-20 carbon atoms, phenyl, alkoxyphenyl, wherein the alkyl group is of 1-10 carbon atoms, and alkylphenyl, wherein the alkyl group is of 1-10 carbon atoms;
- and R.sup.70 is a group of Formula XI
- --(C.dbd.O)R.sup.80 XI
- wherein R.sup.80 is selected from the group consisting of alkyl of 1-20 carbon atoms, phenyl, benzyl, phenyl substituted at any one position with alkyl of 1-10 carbon atoms, chloro, bromo, nitro, or cyano, and benzyl substituted at any one position on the phenyl ring with alkyl of 1-10 carbon atoms, chloro, bromo, nitro or cyano.
3 more elements
Dependencies are inferred from the claim text.