# US 5,132,220 A · More virulent biotype isolated from wild-type virus

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- Number: US 5,132,220 A
- Document ID: US5132220A
- Status: Granted
- Kind: A
- Application number: 07/373,977
- Filed: 1989-06-30
- Granted: 1992-07-21
- Earliest claimed: 1989-06-30
- Assignees: The United States of America as represented by the Secretary of Agriculture
- Inventors: Martin Shapiro; Dwight E. Lynn; Edward M. Dougherty
- Web page: https://patentgrep.com/patent/US5132220A

## CPC

- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- C12N 2710/14021: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A method has been found to select the most virulent biotypes from a wild-type entomopathogenic virus by selection of desirable characteristics after serial passages through in vivo and in vitro systems. Using this method, a strain of Lymantria dispar NPV virus was isolated which had increased biological activity and increased speed of kill.

## Claims

### Claim 1

A biologically pure culture of an entomopathogenic viral strain having all the identifying characteristics of ATCC Accession No. VR 2243.