Isolation of stearidonic acid from fatty acid mixtures
AssigneeNestec S.A.
InventorsMichel Lagarde, Helmut Traitler, Hans-Juergen Wille
Life of this patent
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Abstract
Substantially pure stearidonic acid is isolated from a mixture of polyunsaturated fatty acids by fractionating at 25% to 35% by weight solution of fatty acids by high-performance reverse-phase liquid chromatography using a mobile phase of 75% to 95% by weight methanol and 25% to 5% by weight water. The isolated stearidonic acid is used to prepare pharmaceutical compositions which are administered to treat cardiovascular and thrombo-embolic diseases associated with platelet aggregation.
Claims20
A process for obtaining stearidonic acid from a mixture of fatty acids comprising reacting a mixture of fatty acids containing stearidonic acid with urea and collecting a fraction enriched with polyunsaturated fatty acids, preparing a 25% to 35% by weight solution of fatty acids obtained from the fraction in a solvent, injecting a sample of the solution into a high-performance reverse-phase liquid chromatography column having a porous silica stationary phase adsorbent doped with a layer of saturated hydrocarbons, passing a mobile phase of 75% to 95% by weight methanol and 25% to 5% by weight water through the column, and collecting a fraction containing stearidonic acid.
A pharmaceutical composition in unit dosage form comprising a fatty acid fraction comprised of stearidonic acid in an amount of more than 90% by weight of the fraction.
A method for treating cardiovascular and thrombo-embolic diseases associated with platelet aggregation comprising administering 0.1 g to 1 g stearidonic acid per day in unit dosage form.
Dependencies are inferred from the claim text.