Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens

Essentially pure toxin, active against lepidopteran insects, having the amino acid sequence shown in FIG. 1.

A recombinant DNA transfer vector comprising DNA having the nucleotide sequence which codes for the amino acid sequence shown in FIG. 1.

A bacterial host transformed to express a B.t. toxin having the amino acid sequence shown in FIG. 1.

An insecticidal composition comprising insecticide containing substantially intact, treated cells having prolonged pesticidal activity when applied to the environment of a target pest, wherein said insecticide is a polypeptide toxic to lepidopteran insects, is intracellular, and is produced as a result of expression of a transformed microbe capable of expressing the B.t. toxin having the amino acid sequence shown in FIG. 1.

Treated, substantially intact unicellular microorganism cells containing an intracellular toxin, which toxin is a result of expression of a Bacillus thuringiensis toxin gene toxic to lepidopteran insects which codes for a polypeptide toxin having the amino acid sequence shown in FIG. 1, wherein said cells are treated under conditions which prolong the insecticidal activity when said cell is applied to the environment of a target insect.

The cells, according to claim 22, wherein the cells are treated by chemical or physical means to prolong the insecticidal activity in the environment.

A plasmid selected from the group consisting of pKK2, pKK73BB-9, pKKl-73, pl,123-1, pl,130-6 and pM2,16-11.