Molecules with antibody combining sites that exhibit catalytic properties
AssigneeScripps Clinic and Research Foundation
InventorsRichard A. Lerner, Kim Janda, Diane Schloeder
Life of this patent
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Abstract
An analog-ligand having a conformation that substantially corresponds to the conformation of a hydrolytic transition state of an amide or ester reactant ligand is used to produce receptor molecules of predetermined specificity. The receptor molecules include an antibody combining site that binds to the analog-ligand and also to a reactant ligand and thereby stabilizes the tetrahedral carbon atom of the amide or ester hydrolysis transition state of that reactant ligand to catalytically hydrolyze the reactant ligand at a predetermined site.
Claims2
Monoclonal antibody molecules or antibody combining site-containing portions thereof that catalytically hydrolyze a preselected amide bond of a reactant ligand, said antibody combining site binding:
- (a) to said reactant ligand having said preselected amide bond;
- and
- (b) to an analog of said reactant ligand having a tetrahedrally bonded phosphorus atom located at the position occupied by the carbonyl carbon of said preselected amide bond of said reactant ligand, said tetrahedrally bonded phosphorus atom being bonded directly to:
- (i) the alpha-carbon atom of the acid portion of said analogous ligand amide;
- (ii) two oxygen atoms, on of which is doubly bonded to said phosphorus atom, and the other of said two oxygen atoms is bonded singly to said phosphorus and singly to a radical selected from the group consisting of hydrogen and C.sub.1 -C.sub.4 lower alkyl;
- and
- (iii) a nitrogen atom that is bonded to the alpha-carbon of the amine portion of said analogous ester or amide.
Dependencies are inferred from the claim text.