US 5,120,643 A · Process for immunochromatography with colloidal particles

Abstract

The present invention relates to improved specific binding assay methods, kits and devices utilizing chromatographically mobile specific binding reagents labelled with colloidal particles. Specific binding reagents labelled with colloidal particles such as gold and selenium may be subjected to rapid chromatographic solvent transport on chromatographic media by means of selected solvents and chromatographic transport facilitating agents. Further, impregnation of solid substrate materials with labile protein materials including colloidal particle and enzyme labelled reagents in the presence of meta-soluble proteins provides for the rapid resolubilization of such materials which have been dried onto such substrate materials.

Claims31

Dependent claims

A method for determining the presence or amount of an analyte in a test sample, comprising the steps of:

a) contacting a chromatographic medium with the test sample, said medium comprising at least two reaction sites,
a first reaction site comprising a dried solution of a labelled specific binding reagent in the presence of a meta-soluble protein, and
a second reaction site comprising an immobilized specific binding reagent capable of immobilizing said labelled reagent in relation to the presence or amount of the analyte in the test sample;
b) solubilizing said labelled reagent and transporting at least a portion of said labelled reagent to said second reaction site, and binding said labelled reagent to said second reaction site in relation to the presence or amount of the analyte in the test sample;
and
c) detecting said labelled reagent at said second site to determine the presence or amount of the analyte in the test sample.

3 more elements

Dependencies are inferred from the claim text.

Process for immunochromatography with colloidal particles

Life of this patent

Process for immunochromatography with colloidal particles

Life of this patent

Abstract

Claims31