Microbial cyanide converting enzymes, their production and use
AssigneeNovo Industri A/S
InventorsKjeld Ingvorsen, Sven E. Godtfredsen, Birgitte Hojer-Pedersen
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Abstract
A novel cyanide converting enzyme, a "cyanidase" is described. The enzyme is extremely efficient in reducing substantial concentrations of cyanide to very low levels in a broad pH, and temperature range, and in the presence of organics and metal ions.
Claims20
Cyanidase, a cyanide converting enzyme of bacterial origin which converts inorganic cyanide to formic acid and ammonia, said enzyme being characterized by enzymatic activity in the presence of sodium cyanide at a concentration above about 0.1M and by an enzymatic capability to reduce inorganic cyanide to below about 1 ppm.
A cyanide converting enzyme elaborated by the microorganism of the Accession No. DSM 4009.
A cyanide converting enzyme elaborated by the microorganism of the Accession No. DSM 4010.
A process for the production of a cyanidase by cultivating a cyanidase producing strain of Alcaligenes denitrificans subspecies denitrificans in the presence of a source of assimilable nitrogen, carbon and oxygen, plus essential nutrients, and subsequently recovering the cyanidase, said cyanidase being a cyanide converting enzyme which converts inorganic cyanide to formic acid and ammonia, said enzyme being characterized by enzymatic activity in the presence of sodium cyanide at a concentration above about 0.1M and by an enzymatic capability to reduce inorganic cyanide to below about 1 ppm.
A method for detoxification of cyanide containing streams which comprises contacting such a stream with a cyanidase which converts inorganic cyanide to formic acid and ammonia, said enzyme being characterized by enzymatic activity in the presence of sodium cyanide at a concentration above about 0.1M and by an enzymatic capability to reduce inorganic cyanide to below about 1 ppm.
Dependencies are inferred from the claim text.