Preparation of islet amyloid polypeptides (IAPP) and antibodies to IAPP
AssigneeRegents of the University of Minnesota
InventorsPer Westermark, Kenneth H. Johnson
Life of this patent
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Abstract
Subunits of the full length 37 amino acid residue human Islet Amyloid Polypeptide, and feline Islet Amyloid Polypeptide essentially free of unpolymerized amyloid are provided. Islet Amyloid Polypeptide (IAPP) may be isolated and purified from amyloid fibrils using depolymerizing agent and chromatographic techniques. The sequences of the purified Islet Amyloid Polypeptides have been determined Purified Islet Amyloid Polypeptides are suitable for induction of anti-IAPP antibodies.
Claims4
A polypeptide consisting essentially of:
- Asn-Thr-Ala-Thr-Cys-Ala-Thr-Gln-Arg-Leu-Ala-Asn-Phe-Leu-Val-His-Ser-Ser-As n-Asn-Phe-Gly-Ala-Ile-Leu-Ser-Ser-Thr-Asn-Val-Gly-Ser-Asn.
A polypeptide consisting essentially of:
- Ala-Thr-Gln-Arg-Leu-Ala-Asn-Phe-Leu-Val-His-Ser-Ser-Asn-Asn-Phe-Gly-Ala-Il e-Leu-Ser-Ser-Thr-Asn-Val-Gly.
A polypeptide consisting essentially of:
- Cys-Ala-Thr-Gln-Arg-Leu-Ala-Asn-Phe-Leu-Ile.
A polypeptide consisting essentially of:
- Lys-Cys-Asn-Thr-Ala-Thr-Cys-Ala-Thr-Gln-Arg-Leu-Ala-Asn-Phe-Leu-Ile-Arg-Se r-Asn-Asn-Leu-Gly-Ala-Ile-Leu.
Dependencies are inferred from the claim text.