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US 5,110,908 AGranted
Haemophilus influenzae peptides and proteins
AssigneePraxis Biologics, Inc.
InventorsRobert A. Deich, Gary Zlotnick, Bruce Green
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AssigneePraxis Biologics, Inc.
InventorsRobert A. Deich, Gary Zlotnick, Bruce Green
Estimating the term…
Peptides and proteins related to an epitope comprising an outer membrane protein of Haemophilus influenzae are described. The peptides and proteins can be prepared by methods including novel and improved methods of puiification from H. influenzae cultures, and by recombinant DNA and chemical synthetic techniques. Additionally, recombinant vectors containing nucleotide sequences encoding PBOMP-1 and PBOMP-2 related peptides and proteins are also described. Recombinant vectors include plasmid DNA and viral DNA such as human viruses, animal viruses, insect viruses and bacteriophages that direct the expression of the PBOMP-1 and PBOMP-2 related peptides and proteins in appropriate host cells. The peptides, proteins and viruses both "live" and "inactivated" are used as immunogens in vaccine formulations to protect against H. influenzae infections. The peptides and proteins are also used as reagents in immunoassays as well as to prepare immunoglobulins for passive immunization. Use of the nucleotide sequences encoding the PBOMP related peptides and proteins in hybridization assays is also described.
A substantially pure antigenic peptide or protein of approximately 16000 dalton molecular weight which is PBOMP-1, an Haemophilus influenzae outer membrane protein, said peptide or protein having an amino acid sequence substantially as depicted in FIG. 11 from amino acid residue 20 to 153, in which the peptide or protein is obtained without the use of denaturing detergents.
A substantially pure antigenic peptide or protein of approximately 16000 dalton molecular weight which is PBOMP-2, an Haemophilus influenzae outer membrane protein, said peptide or protein having an amino acid sequence substantially as depicted in FIG. 15 from amino acid residue 19 to 154.
Dependencies are inferred from the claim text.