Purified cyclodextrinase
AssigneeKikkoman Corporation
InventorsTetsuya Oguma, Mamoru Kikuchi, Kiyoshi Mizusawa, Shoichi Tokutake, Nobuyuki Yamaji
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Abstract
The present invention relates to a novel cyclodextrinase having the specific physicochemical characteristics relating to action, substrate specificities, optimum pH, stable pH, inhibition, activation and molecular, weight. This novel enzyme can be produced by culturing a specified microorganism belonging to the genus Bacillus. The invention also provides a process for preparing maltooligosaccharides by acting the present enzyme to cyclodextrins.
Claims3
A purified cyclodextrinase having the physicochemical characteristics of cyclodextrinase produced by the bacteria deposited under FERM BP-2458, wherein
- said enzyme cleaves cyclodextrins to produce maltooligosaccharides corresponding to the polymerization degree of glucose of the cyclodextrins;
- the K.sub.m of said enzyme for cyclodextrins is no higher than the K.sub.m of said enzyme for linear maltooligosaccharides with the same polymerization degree of glucose as cyclodextrins;
- when .beta.-cyclodextrin is used as substrate, the optimum pH is about 8.0 and the stable pH range is 5.5-9.5;
- the optimum working temperature for the present enzyme is 40.degree. C.;
- said enzyme is inactivated by a treatment at a temperature of 55.degree. C. and pH=7.5 for a period of 15 minutes;
- said enzyme is inhibited 90% by Hg.sup.2+, Cu.sup.2+, Zn.sup.2+, Ni.sup.2+ and activated 10-30% by Ca.sup.2+, Mg.sup.2+ ;
- the molecular weight of said enzyme is 144,000 when measured by gel filtration method and 72,000 when measured by SDS PAGE method.
Dependencies are inferred from the claim text.