Selective gene expression in plants
AssigneeThe Rockefeller University
InventorsPhilip N. Benfey, Nam-Hai Chua
Life of this patent
Estimating the term…
Abstract
Fragments of the 35S promoter of cauliflower mosaic virus are disclosed which exhibit selective expression of chimeric plant genes in plant tissue. Promoter fragment A exhibits selective expression in root tissue and the radical of the seed. Promoter fragment B exhibits constitutive expression in plant tissue other than root tissue.
Claims5
In a method for the expression of a chimeric plant gene, the improvement which comprises the use of a tissue-specific promoter fragment which causes tissue-specific expression in leaves, stems, cotyledons, and vascular tissue of the hypocotyl while causing detectable levels of expression in root vascular tissue when operably coupled directly to a DNA segment corresponding to the -72 to +8 promoter fragment of the Cauliflower Mosaic Virus 35S gene, said tissue-specific promoter fragment having the sequence:
- ##STR1##
A chimeric plant gene comprising in sequence in the 5' to 3' direction a tissue-specific promoter fragment consisting essentially of the sequence:
- ##STR2## operably coupled directly to the -72 to +8 promoter fragment of the CaMV 35S gene, said -72 to +8 promoter fragment operably coupled to a structural gene.
A tissue-specific promoter fragment which functions in plants to cause tissue-specific expression in the leaves, stems, cotyledons and the vascular tissue of the hypocotyl and detachable levels of expression in root vascular tissue operably coupled directly to a DNA segment corresponding to the -72 to +8 promoter fragment of the Cauliflower Mosaic Virus 35S gene, said tissue-specific promoter fragment having the sequence from its 5' to 3' termini:
- ##STR3##
Dependencies are inferred from the claim text.