System for biotin synthesis
AssigneeAmgen Inc.
InventorEric F. Fisher
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Abstract
A system for the production of biotin wherein a biotin retention-deficient strain of a cell is transformed with plasmid bearing the biotin gene cluster bio (A, B, F, C and D). The media of cultures of the resulting cells contains enhanced amounts of biotin by comparison with similar constructions in strains capable of biotin retention.
Claims15
A system for the production of biotin comprising:
- an Escherichia coli cell having a biotin retention-deficient mutant genotype:
- and
- nonlytic extrachromosomal DNA, within said cell, encoding at least one gene product of the biotin operon of Escherichia coli or a functional homolog thereof and having a uvrB.sup.- genotype.
A system for the production of biotin comprising:
- an Escherichia coli cell having a (bio.sup.-, birA.sup.-, bioR.sup.-) genotype;
- and
- extrachromosomal DNA, within said cell, encoding the biotin operon of Escherichia coli and having a uvrB.sup.- genotype.
A method for converting an Escherichia coli cell, having a biotin retention deficient mutant genotype to an Escherichia coli cell having enhanced biotin production, comprising the steps of:
- transforming the organism with autonomously replicating, nonlytic extrachromosomal DNA having a uvrB.sup.- genotype and encoding a gene product of the biotin operon or a functional homolog thereof.
A method for converting desthiobiotin to biotin comprising the steps of:
- culturing an Escherichia coli cell, having a biotin retention-deficient mutant genotype and having nonlytic extrachromosomal DNA with a uvrB.sup.- genotype encoding a bioB gene product of the biotin operon of Escherichia coli or a functional homolog thereof, in a medium comprising desthiobiotin.
Dependencies are inferred from the claim text.