Method for providing chemically or biologically useful moiety from water-compatible reducible compound
AssigneeEastman Kodak Company
InventorsAlbert J. Mura, Robert T. Belly, Vanessa R. Lum
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Abstract
Certain water-compatible reducible compounds are useful in analytical compositions and elements for assay of various analytes, e.g. microorganisms. These compounds comprise a moiety which provides a detectable species (e.g. a dye) when released from the compound at physiological pH. Further, these compounds are aromatic derivatives or quinones having water-compatibilizing substituents which allow them to be used in compositions without the use of surfactants.
Claims10
A method for providing a chemically or biologically useful moiety comprising, at a pH of 9 or less, reducing a water-compatible reducible compound of the structure CAR--(--R.sup.1).sub.n wherein CAR-- is a substituted or unsubstituted aromatic or quinone nucleus, R.sup.1 is a moiety which comprises a shiftable detectable species, and n is 1 or 2,
- provided said reducible compound is constructed such that it is capable of being reduced at said pH to release said shiftable detectable species, and said reducible compound comprises at least one water-compatibilizing moiety which has a hydrophobic parameter less than about -2.0 and which is selected from the group consisting of carboxy, sulfo, ammonium, sulfonamido, hydroxy, iodoxy and glucosyl, and
- further provided that when R.sup.1 is replaced with H, CAR-- (--H).sub.n is constructed such that it has an E.sub.1/2 of at least about +100 mV when measured in water.
Dependencies are inferred from the claim text.