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US 5,104,788 AGranted
Method of preparing neocytes and gerocytes in a closed system
AssigneeMiles Inc.
InventorsCarmen, Raleigh A., Randy B. Garcez, Barry S. Leng
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Abstract
A method of preparing neocytes and gerocytes in a closed blood bag system useful for the filtration of leukocytes from a red blood cell concentrate followed by the preparation and long term storage of neocytes and, preferably, gerocytes from the filtered concentrate. In very preferred embodiments, the system also provides containers for the expression of plasma and the preparation and long term storage of platelets.
Claims5
A method of preparing neocytes and gerocytes in a closed system comprising the steps of
- (a) introducing a suspension of packed red blood cells and white blood cells into a filter having an entrance port and an exit port and capable of removing substantially all white blood cells from the suspension;
- (b) collecting the filtered red blood cells into a separation bag constructed so as to separate red blood cells into neocytes and gerocytes;
- (c) separating the cells of step (b) into neocytes and gerocytes;
- (d) expressing the neocytes into a first bag in direct sterile communication with the separation bag and containing a solution selected from a saline solution and a red blood cell storage solution;
- (e) reconstituting the gerocytes of step (c) with a solution from a second bag in direct sterile communication with the separation bag;
- and
- (f) expressing the reconstituted gerocytes into the second bag, steps (a) through (f) all being accomplished within a closed blood bag system.
4 more elements
Dependencies are inferred from the claim text.