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AssigneeDNA Plant Technology Corporation
InventorsWilliam T. Tucker, Neal I. Gutterson
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This invention relates to a new method for inserting heterologous genes into the genome of a bacteria using a combined plasmid. The combined plasmid provides a cis complementation of transposase genes and transposable elements. The method involves the homologous recombination of a carrier plasmid and a functions plasmid to form the combined plasmid. The carrier plasmid contains a transposable element which flanks a generic expression cassette. The functions plasmid comprises transposase genes which complement the transposable element on the carrier plasmid. The combined plasmid is then transferred to a recipient and the recipient is monitored for integration of the generic expression cassette into the genome. The combined plasmid is preferably created by an in vivo homologous recombination of the carrier and functions plasmids.
A method for the insertion of foreign DNA into the genome of a recipient bacterium by introduction of a combined plasmid containing a transposable cassette and a transposase encoding sequence which is competent to transpose the cassette, said method comprising the steps of forming the combined plasmid from a carrier plasmid and a functions plasmid by homologous recombination wherein the recombination occurs between overlapping segments of a sselectable marker such that recombination results in the construction of a functional selectable marker.
A method for the insertion of foreign DNA into the chromosome of a recipient bacterium which comprises the steps of:
A system for the insertion of foreign DNA into the genome of a recipient bacterium wherein the recipient bacterium receives a combined plasmid from a donor bacterium, said system comprising:
Dependencies are inferred from the claim text.