Cloning of the bioA, bioD, bioF, bioC and BioH genes of bacillus spraericus, vectors and transformed cells
AssigneeTransgene S.A.
InventorsRemi Gloeckler, Denis Speck, Yves Lemoine
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Abstract
The present invention relates, in particular, to a DNA sequence corresponding to one of the following genes involved in the chain of biotin biosynthesis in bacteria: bioA gene, bioD gene, bioF, bioC and bioH gene. Vectors containing these sequences enable other microorganisms to be transformed in order to improve the production of biotin.
Claims21
A recombinant DNA molecule comprising a sequence encoding a gene product of B. sphaericus, wherein said sequence is selected from the group consisting of:
- (i) a sequence encoding the product of gene bio A, (ii) a sequence encoding the product of gene bioD, (iii) a sequence encoding the product of gene bio F, (iv) a sequence encoding the product of gene bio H, (v)( a sequence encoding the product of gene bio X, (vi) a sequence encoding the product of gene bio Y, and (viii) a sequence encoding the product of gene bio W.
A recombinant DNA molecule comprising, in any order, (i) a first open reading frame (ORF) encoding the product of gene bio D of B. sphaericus, (ii) a second ORF encoding the product of gene bio A of B. sphaericus, (iii) a third ORF encoding the product of gene bio Y of B. sphaericus and (iv) a fourth ORF encoding the product of gene bio B of B. sphaericus.
A recombinant DNA molecule comprising, in any order, (i) a first ORF encoding the product of gene bio X of B. sphaericus, (ii) a second ORF encoding the product of gene bio W of B. sphaericus, and (iii) a third ORF encoding the product of gene bio F of B. sphaericus.
A plasmid selected from pTG1400 and pTG1418.
Dependencies are inferred from the claim text.