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US 5,096,819 AGranted
Hyperproduction of poly-.beta.-hydroxybutyrate during exponential growth by mutant strains of Azotobacter vinelandii
AssigneeThe Governors of the University of Alberta
InventorsWilliam J. Page, Olga Knosp
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Abstract
A mutant strain of Azotobacter vinelandii exhibits hyperproduction of poly-.beta.-hydroxybutyrates during its growth. Poly-.beta.-hydroxybutyrate yield is further enhanced when sugar beet molasses is used as carbon source.
Claims4
A process for producing pHB comprising:
- (i) culturing under intense aeration conditions mutant bacteria of the species Azotobacter vinelandii, said mutant having the identifying characteristics of ATCC 53799, in a culture medium having a source of assimilable nutrients comprising unrefined carbon sources which are of a type suitable to maintain said bacteria and to induce exponential bacteria growth, wherein said mutant bacteria hyperproduce pHB;
- and
- (ii) recovering pHB from said bacteria.
Dependencies are inferred from the claim text.