Process and reagent for the improved quantitative colorimetic determination of hydrogen peroxide
AssigneeBoehringer Mannheim GmbH
InventorsJoachim Siedel, Albert Roder, Joachim Ziegenhorn
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Abstract
The present invention provides a process for the improved colorimetric determination of hydrogen peroxide as formed by a hydrogen peroxide-producing oxidase, by addition of a chromogenic system and measurement of the colored material formed, wherein superoxide dismutase (E.C. 1.15.1.1) is added to the reagent solution. The present invention also provides a reagent for the improved colorimetric determination of hydrogen peroxide, comprising a hydrogen peroxide-producing oxidase, a chromogenic system, a buffer and optionally adjuvant enzymes, wherein it also contains superoxide dismutase.
Claims20
A process for colorimetric determination of hydrogen peroxide formed by a hydrogen peroxide producing oxidase which does not produce superoxide comprising adding to a non-superoxide containing sample containing said hydrogen peroxide producing oxidase a chromogenic system and superoxide dismutase in an amount sufficient to increase sensitivity of colorimetric determination of said chromatogenic system.
A reagent for colorimetric determination of hydrogen peroxide comprising a hydrogen peroxide-producing oxidase, a chromogenic system, a buffer optionally adjuvant enzymes, and superoxide dimutase in an amount effective to improve the sensitivity of the colorimetric determination of the chromogenic system.
Dependencies are inferred from the claim text.