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US 5,093,268 AGranted
Apparatus for conducting a plurality of simultaneous measurements of electrochemiluminescent phenomena
AssigneeIgen, Inc.
InventorsNicholas Leventis, Susan E. Morris
Life of this patent
Estimating the term…
Abstract
An apparatus for conducting two or more simultaneous measurements of electrochemiluminescent (ECL) phenomena includes a cell in which a sample including an ECL moiety may be induced to repeatedly emit light in response to electrochemical energy applied by, for example, a working voltammetric electrode. Two or more light detectors such as photomultiplier tubes receive the emitted light at respective wavelengths indicative of respective analytes of interest in the sample.
Claims21
An apparatus for conducting two simultaneous measurements of electrochemiluminescent phenomena, comprising:
- (a) electrochemical cell means for holding a sample therein, said cell means including a sample-holding volume and first and second transparent plates at least partly defining said sample-holding volume therebetween;
- (b) electrode means including at least a working electrode and a counter electrode for triggering electrochemiluminescence in a sample held within said sample-holding volume, said working electrode being mounted on at least one of said first and second transparent plates so as to be exposable to said sample and further being substantially transparent at a plurality of electrochemiluminescent wavelengths and being configured so as to trigger said sample to emit electrochemiluminescent radiation towards said first and second transparent plates;
- and
- (c) first light detection means having a light receiving surface confronting said first transparent plate for detecting radiation at a first one of said electrochemiluminescent wavelengths and second light detection means having a light receiving surface confronting said second transparent plate for detecting radiation at a second one of said electrochemiluminescent wavelengths, said first and second light detection means being independently operable and adapted for simultaneous operation for light detection at the first and second wavelengths, respectively.
1 more element
An apparatus for conducting a plurality of simultaneous measurements of electrochemiluminescent phenomena comprising:
- (a) electrochemical cell means for holding a sample therein, said cell means including a sample-holding volume and first and second transparent plates at least partly defining said sample-holding volume therebetween;
- (b) electrode means including at least a working electrode and a counter electrode for triggering electrochemiluminescence in a sample held within said sample-holding volume, said working electrode being mounted on at least one of said first and second transparent plates so as to be exposable to said sample and further being substantially transparent at a plurality of electrochemiluminescent wavelengths and being configured so as to trigger said sample to emit electrochemiluminescent radiation towards said first and second transparent plates;
- and
- (c) a plurality of light detection means having respective light receiving surfaces confronting ones of said first and second transparent plates for detecting light at respective ones of said electrochemiluminescent wavelengths, said plurality of light detection means being independently operable and adapted for simultaneous operation for light detection at the respective wavelengths.
1 more element
An apparatus for conducting a plurality of measurements of electrochemiluminescent phenomena comprising:
- (a) electrochemical cell means for holding a sample therein, said cell means including a sample-holding volume;
- (b) electrode means including at least a working electrode and a counter electrode for triggering electrochemiluminescence in a sample held within said sample-holding volume, said working electrode being mounted in said sample-holding volume so as to be exposable to said sample;
- (c) first and second light detection means for detecting light of at least first and second electrochemiluminescent wavelengths, respectively;
- and
- (d) means interposed between said first and second light detection means and said cell means for transmitting light at said first and second electrochemiluminescent wavelengths from said sample to said light detection means.
2 more elements
An apparatus for conducting two simultaneous measurements of electrochemiluminescent phenomena, comprising:
- (a) electrochemical cell means for holding a sample therein, said cell means including opposed first and second transparent plates defining a sample-holding volume therebetween;
- (b) electrode means mounted in said cell means and including at least a working electrode and a counter electrode for triggering electrochemiluminescence in a sample held within said sample-holding volume, said working electrode being mounted on at least one of said first and second transparent plates so as to be exposable to said sample and further being substantially transparent at a plurality of electrochemiluminescent wavelengths and being configured so as to trigger said sample to emit electrochemiluminescent radiation towards said first and second transparent plates;
- (c) cell holding means for removably holding said cell means at an operative position within said apparatus;
- (d) voltage control means adapted to be electrically connected to said electrode means when said cell means is held at said operative position for applying voltage signals to said electrode means;
- (e) first light detection means for detecting light at a first one of said electrochemiluminescent wavelengths, said first light detection means including first filter means having a respective light receiving surface directly confronting said first transparent plate for transmitting light at said first one of said electrochemiluminescent wavelengths and photomultiplier tube means directly confronting said first filter means for detecting light transmitted by the latter;
- and
- (f) second light detection means for detecting light at a second one of said electrochemiluminescent wavelengths, said second light detection means including second filter means having a respective light receiving surface directly confronting said second transparent plate for transmitting light at said second one of said electrochemiluminescent wavelengths and second photomultiplier tube means directly confronting said second filter means for detecting light transmitted by the latter;
- (g) said first and second light detection means being independently operable, whereby said apparatus is adapted for light detection at the first and second wavelengths by said first and second light detection devices, respectively, to conduct two simultaneous measurements of electrochemiluminescent phenomena.
5 more elements
A method for conducting a plurality of simultaneous measurements of electrochemiluminescent phenomena, comprising the steps of:
- (a) selecting a sample including at least first and second electrochemiluminescent moieties which are respectively electrochemically inducible to emit light at respective first and second electrochemiluminescent wavelengths;
- (b) exposing said sample to a working electrode adapted to induce the emission of light in said sample;
- (c) applying a voltage signal to said working electrode to induce the emission of light at said first and second electrochemiluminescent wavelengths;
- (d) detecting light at said first electrochemiluminescent wavelength using a first light detection means;
- and
- (e) simultaneously detecting light at said second electrochemiluminescent wavelength using a second light detection means.
3 more elements
A method for conducting a plurality of measurements of electrochemiluminescent phenomena, comprising the steps of:
- (a) selecting a sample including at least first and second electrochemiluminescent moieties which are respectively electrochemically inducible to emit light at respective first and second electrochemiluminescent wavelengths;
- (b) exposing said sample to a working electrode adapted to induce the emission of light in said sample;
- (c) applying a voltage signal to said working electrode to induce the emission of light at said first and second electrochemiluminescent wavelengths;
- (d) detecting light at said first electrochemiluminescent wavelength using a first light detection means;
- and
- (e) detecting light at said second electrochemiluminescent wavelength using a second light detection means.
3 more elements
Dependencies are inferred from the claim text.