Nucleic acid probes
AssigneeChiron Corporation
InventorsMichael S. Urdea, Thomas Horn
Life of this patent
Estimating the term…
Abstract
Modified nucleotides are provided which have the structure ##STR1## wherein R.sup.1 is a reactive group derivatizable with a detectable label, R.sup.2 is an optional linking moiety including an amide, thioether or disulfide linkage or a combination thereof, R.sup.3 is hydrogen, methyl, bromine, fluorine or iodine, R.sup.4 is hydrogen, an acid-sensitive, base-stable blocking group of an acyl capping group, R.sup.5 is hydrogen or a phosphorus derivative, R.sup.6 is H, OH, or OR where R is a protecting group and x is an integer in the range of 1 and 8 inclusive. Methods of synthesizing the derivatizable nucleotide are disclosed, as are labeled polynucleotide probes prepared therefrom.
Claims14
A modified nucleotide having the structure ##STR22## wherein:
- R.sup.1 is a protected or unprotected --NH.sub.2, --COOH or --SH group;
- R.sup.2 is a covalent bond or an amide linking moiety with the priviso that the amide linkage is oriented --NH--C(.dbd.O)--;
- R.sup.3 is hydrogen, methyl, bromine, fluorine or iodine;
- R.sup.4 is selected from the group consisting of hydrogen, acid-sensitive, base-stable blocking groups and acyl capping groups;
- R.sup.5 is a phosphorus derivative selected from the group consisting of PO.sub.3 H.sub.2, phosphotriesters, phosphodiesters, phosphites, phosphoramidites, H-phosphonates and phosphorothioates;
- R.sup.6 is H, OH, or OR where R is an acid-sensitive, base-stable protecting group;
- and
- x is an integer in the range of 1 and 8 inclusive,
- wherein the nucleotide may bound through R.sup.5 to a solid support.
Dependencies are inferred from the claim text.