Polymorphism of human platelet membrane glycoprotein IIIa and diagnostic and therapeutic applications thereof
AssigneeThe Blood Center of Southeastern Wisconsin
InventorsPeter J. Newman, Richard H. Aster
Life of this patent
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Abstract
Isolated polynucleotide molecules, and peptides encoded by those molecules, can be used in the analysis of alloantigen phenotypes, as well as in diagnostic and therapeutic applications relating to human platelet Pl.sup.A polymorphism. In this vein, a method for typing blood cell and platelet membrane glycoproteins entails an analysis of amplified cDNA, encoded by platelet and red blood cell mRNA.
Claims26
A method of typing human platelet glycoproteins, comprising the steps of
- (a) synthesizing cDNA from human platelet;
- (b) amplifying said cDNA to produce amplified cDNA;
- and then
- (c) analyzing said amplified cDNA to determine a paltelet phenotype.
A method as claimed in cailm 3 wherein (i) said cDNA comprises a sequence that encodes at least nucleotide 196 of CPIIIa mRNA and (ii) said restriction endonuclease recognizes a restriction site, which comprises or is adjacent to a nucleotide sequence that includes nucleotide 196 of CPIIIa mRNA.
A method of typing platelet Pl.sup.A membrane glycoproteins comprising the steps of (a) obtaining genomic DNA from a first individual and (b) analyzing said genomic DNA to determine a platelet Pl.sup.A alloantigen phenotype.
A method of typing platelets with respect to GPIIIA comprising the steps of (a) obtaining genomic DNA from an first individual, (b) amplifying said genomic DNA to produce amplified genomic DNA and (c) analyzing said amplified genomic DNA to determine a platelet Pl.sup.A alloantigen phenotype.
A kit for typing platelet Pl.sup.A alloantigens, comprising
- (a) means for amplifying DNA that comprises at least a portion of a GPIIIA gene or GPIIIA cDNA, wherein said portion includes a nucleotide corresponding to nucleotide 196 of said GPIIIA cDNA, and at least one component selected from the group consisting of
- (b) a receptacle containing a solution of a labeled oligonucleotide probe that distinguishes a Pl.sup.A allele from another Pl.sup.A allele, and
- (c) a receptacle containing a solution of an endonuclease recognizing a cleavage site that distinguishes a nucleotide sequence of a Pl.sup.A allele from another Pl.sup.A alleles.
A kit for typing platelet Pl.sup.A alloantigens, comprising a receptacle containing a solution of an antibody that discriminately binds alloantigens expressed by a Pl.sup.A1 allele or a Pl.sup.A2 allele of GPIIIA, said antibody (i) recognizing a peptide molecule encoded by a nucleotide sequence encoding at least amino acid 33 of GPIIIA and (ii) binding either said Pl.sup.A1 allele or said Pl.sup.A2 allele of GPIIIA.
Dependencies are inferred from the claim text.