Polysaccharides and antiviral drugs containing the same as active ingredient
AssigneeKureha Kagaku Kogyo Kabushiki Kaisha
InventorsShigeaki Muto, Koichi Niimura, Minoru Oohara, Yoshiharu Oguchi, Kenichi Matsunaga, Kunitaka Hirose, Junji Kakuchi, Norifumi Sugita, Takao Furusho, Chikao Yoshikumi, Masaaki Takahashi
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Abstract
Disclosed herein are polysaccharides extracted from marine algae and an antiviral drug containing as active ingredient polysaccharides extracted from seaweeds.
Claims14
A protein-bound polysaccharide, having antiviral activity, obtained from a marine alga belonging to the genera Nemacystus, Kjellmaniella, Laminaria, Undaria, Hizikia, Porphyra, Gelidium, Gloiopeltis, Gracilaria, Hemineura, Ulva, Spirogyra, Codium and Acetabularia having the following properties:
- (a) positive results when subjected to the .alpha.-naphthol-sulfuric acid, indole-sulfuric acid, anthrone-sulfuric acid or phenol-sulfuric acid reaction and, after hydrochloric acid hydrolysis, a Lowry-Folin process or ninhydrin reaction;
- (b) elementary analysis:
- 20-55% carbon, 3-9% hydrogen and less than 16% nitrogen;
- (c) a pH of 6.0-7.5;
- (d) the sugar component comprises at least two kinds of saccharides selected from glucose, glucuronic acid, xylose and mannose, and the protein component comprises at least three kinds of amino acids selected from aspartic acid, lysine, leucine, glutamic acid and glycine;
- (e) infrared absorption spectrum peaks at 3,600-3,200 cm.sup.-1 and 1,700-1,600 cm.sup.-1 ;
- (f) a molecular weight of 10.sup.3 -3.times.10.sup.6 as measured by gel filtration chromatography;
- and
- (g) soluble in water or aqueous solvents containing water-soluble alcohols, acids or bases but insoluble in chloroform, benzene and ether.
A pharmaceutical composition comprising, together with a pharmaceutically acceptable carrier or diluent, as an active ingredient, an effective amount of an antiviral protein-bound polysaccharide extracted from a marine alga belonging to genera Nemacystus, Kjellmaniella, Laminaria, Undaria, Hizikia, Porphyra, Gelidium, Gloiopeltis, Gracilaria, Hemineura, Ulva, Spirogyra, Codium and Acetabularia, said protein-bound polysaccharide having the following properties:
- (a) positive results when subjected to the .alpha.-naphthol-sulfuric acid, indole-sulfuric acid, anthrone-sulfuric acid or phenol-sulfuric acid reaction and, after hydrochloric acid hydrolysis, a Lowry-Folin process or ninhydrin reaction;
- (b) elementary analysis:
- 20-55% carbon, 3-9% hydrogen and less than 16% nitrogen;
- (c) a pH of 6.0-7.5;
- (d) the sugar component comprises at least two kinds of saccharides selected from glucose, glucuronic acid, xylose and mannose, and the protein component comprises at least three kinds of amino acids selected from aspartic acid, lysine, leucine, glutamic acid and glycine;
- (e) infrared absorption spectrum peaks at 3,600-3,200 cm.sup.-1 and 1,700-1,600 cm.sup.-1 ;
- (f) a molecular weight of 10.sup.3 - 3.times.10.sup.6 as measured by gel filtration chromatography;
- and
- (g) soluble in water or aqueous solvents containing water-soluble alcohols, acids or basis but insoluble in chloroform, benzene and ether.
Dependencies are inferred from the claim text.