Coenzyme-independent L-sorbosone dehydrogenase from Pseudomonas putida
AssigneeHoffmann-La Roche Inc.
InventorsAkiko Fujiwara, Tatsuo Hoshino
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Abstract
A coenzyme-independent L-sorbosone dehydrogenase has been prepared from a microorganism belong to the genus Pseudomonas, which acts on L-sorbosone to produce 2-keto-L-gulonic acid. The enzyme is membrane bound and has a molecular weight of about 47,000 kDa. The enzyme is used to carry out the conversion of L-sorbosone to 2-keto-L-gulonic acid.
Claims6
An enzyme wherein said enzyme is a homogenous protein having the L-sorbosone dehydrogenase activity for converting L-sorbosone to 2-keto-L-gulonic acid in the presence of an electron acceptor without the need for a coenzyme where said protein has a molecular weight of 47,000.+-.5,000 as measured by sodium dodecyl sulfate polyacrylamide gel electrophoresis with said activity of said protein being optimal at a pH of from about 7 to about 8 and at a temperature of from about 20.degree. C. or to about 40.degree. C.
A process for producing 2-KGA comprising oxidizing L-sorbosone in the presence of an enzyme, said enzyme being a homogeneous protein having the L-sorbosone dehydrogenase activity for converting L-sorbosone to 2-Keto-L-gulonic acid in the presence of an electron acceptor without the need for a coenzyme where said protein has a molecular weight of 47,000.+-.5,000 as measured by sodium dodecyl sulfate polyacrylamide gel electrophoresis with said activity of said protein being optimal at a pH of from about 7 to about 8 and at a temperature of from about 20.degree. C. or to about 40.degree. C.
Dependencies are inferred from the claim text.