Probes for the specific detection of Escherichia coli and Shigella
AssigneeGene-Trak Systems
InventorsKyriaki Parodos, Hsien-Yeh Hsu, David Sobell, Janice M. McCarty, David J. Lane
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Abstract
Nucleic acid probes capable of specifically hybridizing to rRNA of E. coli and Shigella species and not to rRNA of non-E. coli/Shigella are described along with methods utilizing such probes for the specific detection of E. coli and/or Shigella in food and other samples.
Claims10
A nucleic acid fragment capable of hybridizing, under predetermined stringency conditions, to rRNA or rDNA (rRNA gene) of E. coli Shigella and not to rRNA or DNA of non-E. coli/Shigella.
An assay kit for detecting E. coli and/or Shigella comprising the nucleic acid fragment of capable of hybridizing under hybridizing conditions to probe 787 packaged in at least one container, and instructions for utilizing said nucleic acid fragment for detecting E. coli and/or Shigella.
A nucleic acid fragment capable of hybridizing under predetermined stringency conditions to rRNA or rDNA (rRNA gene) of E. coli and Shigella and not to rRNA or DNA of non-E. coli/Shigella wherein said fragment is homologous with at least 95l% of a sequence comprising at least any ten consecutive nucleotides within a region of 455 to 477 of the 16S rRNA of E. coli.
Dependencies are inferred from the claim text.