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US 5,082,627 AGranted
Three dimensional binding site array for interfering with an electrical field
AssigneeBiotronic Systems Corporation
InventorWilliam D. Stanbro
Life of this patent
Estimating the term…
Abstract
A dielectric material of a capacitive affinity sensor has a three-dimensional molecular binding site array. A glass base is layered with a binding agent like silane from which a polymeric backbone like polylysine extends. The polymeric backbone is prepared to accept receptor molecules like cortisol hemisuccinate to bind a specific antibody. Such an array changes dielectric properties between the two electrodes of the capacitive affinity sensor to greatly enhance sensitivity of the sensor.
Claims2
An apparatus for detecting an analyte comprising:
- an electrode for generating an electrical field;
- a means for interfering with the electrical field comprising a biochemically active layer that comprises a plurality of polylysine backbones, and a silane for binding each polylysine backbone and extending each polylysine backbone into the electrical field;
- and
- a means for enhancing interference with the electrical field according to a concentration of an analyte, comprising a plurality of receptor molecules biospecific to the analyte and bound to and extending from each polylysine backbone, each receptor molecule being biospecific to the analyte.
1 more element
A capacitive affinity sensor for detecting an analyte comprising:
- a means for generating an electrical field comprising two electrodes having opposite polarities;
- a means for interfering with the electrical field comprising a dielectric material between the two electrodes, the dielectric material comprising a biochemically active layer of a plurality of polylysine backbones and a means for binding each polylysine backbone and extending each polylysine backbone into the electric field and
- a means for enhancing interference with the electrical field according to a concentration of an analyte, comprising a plurality of binding sites on each polylysine backbone, each binding site comprising a receptor molecule biospecific to the analyte and bound to and extending from a polylysine backbone.
Dependencies are inferred from the claim text.