US 5,081,024 A · Process for producing optically active amino acids

Abstract

An efficient process of optical resolution is provided for producing an L-amino acid represented by the following formula (I): ##STR1## wherein R is --CH.sub.2 CO.sub.2 H, --CH.sub.2 CONH.sub.2, --CO.sub.2 H or ##STR2## in which R.sup.1 is hydrogen atom, an alkyl group having 1 to 10 carbon atoms or a halogen-substituted alkyl group having 1 to 10 carbon atoms. The process comprises an optical resolution of an N-substituted carbonyl-D,L-amino acid represented by the formula (II): ##STR3## (wherein R is the same as defined above and R.sup.2 is an alkanoyl group having 1 to 5 carbon atoms, benzoyl group, a halogen-substituted alkanoyl group having 1 to 5 carbon atoms or a halogen-substituted benzoyl group), its salt or a mixture thereof, by means of cells of a microorganism, a fermentation broth of a microorganism, a treated fermentation broth of a microorganism or a mixture thereof, said microorganism being selected from the bacteria of the genera Serratia, Staphylococcus, Bacillus, Flavobacterium, Achromobacter and Alcaligenes and actinomycetes of the genera Actinoplanes, Streptosporangium and Sebekia.

Claims14

Dependent claims

A process for producing an L-amino acid represented by the formula (I):

##STR8## wherein R is --CH.sub.2 CO.sub.2 H, --CH.sub.2 CONH.sub.2, --CO.sub.2 H or ##STR9## in which R.sup.1 is hydrogen atom, an alkyl group having 1 to 10 carbon atoms or a halogen-substituted alkyl group having 1 to 10 carbon atoms, which process comprises optically resolving an N-substituted carbonyl-D,L-amino acid represented by the formula (II);
##STR10## wherein R is as defined above and R.sup.2 is an alkanoyl group having 1 to 5 carbon atoms, a benzoyl group, a halogen-substituted alkanoyl group having 1 to 5 carbon atoms or a halogen-substituted benzoyl group, its salt or a mixture thereof,
by a microorganism, a fermentation broth obtained by cultivating the microorganism, or a mixture thereof, said microorganism being a bacterial strain selected from the group consisting of Serratia mercascens IFO 12648, Staphlococcus aureus IFO 12732, Bacillus brevis NCB 11 (FERM BP-2551), Flavobacterium sp. NCB 12-2 (FERM BP-2552), Achromobacter sp. IFO 13495, Alcaligenes latus NCB 4-12 (FERM BP-2553), Actinoplanes liguriae IFO 13997, Streptosporangium sp. NC 26 (FERM BP-2554), and Sebekia benihana IFO 14309.

Dependencies are inferred from the claim text.

Process for producing optically active amino acids

Life of this patent

Process for producing optically active amino acids

Life of this patent

Abstract

Claims14