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AssigneeRadiometer Corporate Development Ltd.
InventorsAnthony H. Carr, Robert A. Badley, Ian Jobling, Thomas J. Sands
Estimating the term…
A test for microbial growth is carried out by culturing a microorgnism to be tested in a lliquid culture medium containing an enzymatically hydrolyzable fluorogenic substrate for a period of less than about 7 hours, measuring fluorescence of the culture medium resulting from hydrolysis of the fluorogenic substrate by growth of the microorganism, continuing culturing of the microorganism in the culture medium for an additional period, and measuring microorganism growth by a non-fluorescent method as a confirmatory test of growth determined from fluorescence. Preferably, the additional culture period is overnight and the non-fluorescent method is by visual inspection. The culture medium may be in the wall of a microtitre plate, and the culture medium can contain an antibiotic to test for sensitivity of a microorganism to the antibiotic or to test for minimum inhibitory concentration of the antibiotic.
A method for carrying out a microbial culture test comprising innoculating a microorganism under test into a liquid culture medium including at least one enzymatically hydrolysable fluorogenic substrate contained in a well of a microtitre plate cultivating the innoculated microorganism in the liquid culture medium by incubation for a period of less than about 7 hours, thereafter measuring fluorescence yield of the incubated liquid culture, medium resulting from enzymatic hydrolysis of the fluorogenic substrate by microbial growth to detect growth of the microorganism continuing the incubation in the microtitre plate well of the same portion of incubated liquid culture medium on which the fluorescent yield was measured for a further, overnight, culture period to permit further growth of the microorganism under test and effecting a confirmatory test of culture growth of the microorganism by visual inspection for a button of growth in the well.
Dependencies are inferred from the claim text.