Separation of mixtures by two-phase systems
AssigneeLehigh University
InventorJames T. Hsu
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Abstract
An organic two-phase system useful for the separation and purification of chemicals, biochemicals and optical isomers. The two-phase system can be formed with water soluble polymers as one phase, and chiral compound as the other phase together with a water-miscible organic solvent and/or water.
Claims38
A process for separating a mixture of organic compounds, inorganic compounds, biomolecules and biomaterials, comprising the steps:
- a) preparing an organic two-phase system containing a substantial proportion of organic solvent in both phases consisting essentially of 1) a phase forming compound selected from the group consisting of amino acids, peptides, proteins, alditols, inositols, aldonic acids, uronic acids, aldaric acids, monosaccharides, disaccharides, cyclodextrins, chiral salts, chiral solvents, chiral acids, chiral bases and chiral esters 2) a water soluble polymer in amounts sufficient to form said two-phase system and 3) a water-miscible organic solvent selected from the group consisting of dimethylsulfoxide, formamide and ethanolamine;
- b) mixing said two-phase system with said mixture to be separated;
- c) separating the two phases from each other.
A process for separating a mixture of stereoisomers or optical isomers comprising the steps:
- a) preparing an organic tow-phase system containing a substantial proportion of organic solvent in both phases consisting essentially of 1) a phase-forming chiral resolving agent selected from the group consisting of amino acids, peptides, proteins, alditols, inositols, aldonic acids, uronic acids, aldaric acids, monosaccharids, disaccharides, cyclodextrins, chiral salts, chiral solvents, chiral acids, chiral bases and chiral esters 2) a water soluble polymer in amounts sufficient to form said organic two-phase system and 3) a water-miscible organic solvent selected from the group consisting of dimethylsulfoxide, formamide and ethanolamine;
- b) mixing said two-phase system with the mixture of stereoisomers or optical isomers to be separated;
- c) separating the two phases from each other;
- d) recovering a phase which is enriched in one of said optical isomers.
A process for separating a mixture of organic compounds, inorganic compounds, biomolecules and biomaterials, comprising the steps:
- a) preparing a two-phase system containing a substantial proportion of organic solvent in both phases consisting essentially of 1) a phase forming compound selected from the group consisting of glycine, amino acids, peptides, proteins, alditols, inositols, aldonic acids, uronic acids, aldaric acids, monosaccharides, disaccharides, cyclodextrins, chiral salts, chiral solvents, chiral acids, chiral bases and chiral esters 2) a water soluble polymer in amounts sufficient to form said two-phase system and 3) solvent consisting of water and at least one water-miscible organic solvent selected from the group consisting of dimethylsulfoxide, formamide and ethanolamine;
- b) mixing said two-phase system with a mixture to be separated;
- c) separating the two phases from each other.
Dependencies are inferred from the claim text.