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OwnerFrances S. Eigler, Jacque Georger +4inventors
InventorsFrances S. Eigler, Jacque Georger, Suresh K. Bhatia, Jeff Calvert, Lisa C. Shriver-Lake, Reinhard Bredehorst
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Active agents such as proteins are covalently immobilized on substrates carrying hydroxyl groups. A silane is bound to the substrate and coupled to a heterobifunctional crosslinker at one functional group leaving a free functional group, different than the first group, to which a protein is bound while retaining high protein functionality. Preferably, the silane has a functional group which reacts with the hydroxyl group of the substrate and a thiol terminal group which reacts with a functional group of a heterobifunctional crosslinking agent which contains a succinimide group that reacts with an amino group of the active agent. Bound active agents such as proteins are useful as biosensors or reactants in a variety of applications including bioassays.
A method for modifying a substrate to activate said substrate for the immobilization of anzime group-containing active agents, said method comprising,
In a method of immobilizing an amino group-containing active agent on a substrate, the improvement comprising preparing said substrate by,
Dependencies are inferred from the claim text.