Interleukin-1 inhibitors
AssigneeSynergen, Inc.
InventorsCharles H. Hannum, Stephen P. Eisenburg, Robert C. Thompson, William P. Arend, Fenneke G. Joslin
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Abstract
DNA sequences that encode Interleukin-1 inhibitors and recombinant-DNA methods for the production of interleukin-1 inhibitors are provided. The DNA sequences encode proteins having interleukin-1 inhibitors activity.
Claims31
An isolated DNA sequence encoding a physiologically functional interleukin-1 inhibitor (IL-li) comprising a DNA sequence that is selected from the group consisting of (1) a DNA sequence that encodes IL-li X, IL-li alpha, or IL-li beta and (2) a DNA sequence (i) that cross-hybridizes to a DNA sequence that encodes IL-li X, IL-li alpha, or IL-li beta or (ii) that cross-hybridizes to a DNA sequence that is complementary to a DNA sequence that encodes IL-li X, IL-li alpha, or IL-li beta, wherein said DNA sequence of (i) or (ii) encodes a protein having IL-1 inhibitor activity.
The recombinant DNA molecular GT10-lLli-2A.
A recombinant-DNA method for the production of an interleukin-1 inhibitor (IL-li) comprising:
- (a) preparing a DNA sequence encoding a protein having IL-1 inhibitor activity, wherein said DNA sequence is selected from the group consisting of (1) a DNA sequence that encodes IL-li X, IL-li alpha, or IL-li beta and (2) a DNA sequence (i) that cross-hybridizes to a DNA sequence that encodes IL-li X, IL-li alpha, or IL-li beta or (ii) that cross-hybridizes to a DNA sequence that is complementary to a DNA sequence that encodes IL-li X, IL-li alpha, or IL-li beta, wherein said DNA sequence of (i) or (ii) encodes a protein having IL-1 inhibitor activity;
- (b) subcloning the DNA sequence into a vector capable of being inserted into and replicated in a host cell, such vector containing at least one regulatory element needed to express the DNA sequence;
- (c) inserting the vector containing the DNA sequence and at least one regulatory element into a host cell capable of expressing the DNA encoding the IL-1 inhibitor;
- (d) culturing the host cell sunder conditions appropriate for replication of the vector and expression of the IL-1 inhibitor;
- and
- (e) harvesting the IL-1 inhibitor.
Dependencies are inferred from the claim text.