Immobilization of proteins and peptides on insoluble supports

A method of immobilizing a peptide or protein onto a flat, microporous membrane surface, comprising the steps:

• a) adsorbing a peptide or protein onto a polyvinylidene difluoride membrane surface to noncovalently attach the peptide or protein thereto;
• b) depositing a polyamine polymer onto the adsorbed peptide or protein, wherein the polyamine polymer has functional groups which can be crosslinked;
• and
• c) crosslinking the polyamine polymer with 1,4-phenylenediisothiocyanate, to thereby produce a polymer network which entraps the peptide or protein therein, wherein the network does not interfere with subsequent chemical or enzymatic analysis of the protein or peptide.

A method of immobilizing a peptide or protein onto a flat, microporous membrane surface, comprising the steps of:

• a) adsorbing a peptide or protein onto a polyvinylidene difluoride membrane surface to noncovalently attach the peptide or protein thereto;
• b) depositing a polycarboxylic acid polymer onto the adsorbed peptide or protein, wherein the polycarboxylic acid polymer has functional groups which can be crosslinked;
• and
• c) crosslinking the polycarboxylic acid polymer with a dianiline crosslinking agent, to thereby produce a polymer network which entraps the peptide or protein therein, wherein the network does not interfere with subsequent chemical or enzymatic analysis of the protein or peptide.

A method of immobilizing a peptide or protein onto a flat, microporous membrane surface, comprising the steps of:

• a) adsorbing a peptide or protein onto a polyvinylidene difluoride membrane surface to noncovalently attach the peptide or protein thereto;
• b) depositing an azidopolymer or benzophenone polymer onto the adsorbed peptide or protein, wherein the polymer has functional groups which can be crosslinked;
• and
• c) crosslinking the azidopolymer or benzophenone polymer by exposing the microporous membrane to ultraviolet radiation, to thereby produce a polymer network which entraps the peptide or protein therein, wherein the network does not interfere with subsequent chemical or enzymatic analysis of the protein or peptide.

A method of immobilizing a peptide or protein onto a flat, microporous membrane surface, comprising the steps of:

• a) adsorbing a peptide or protein and a polymer onto the microporous membrane surface to noncovalently attach the peptide or protein thereto;
• b) treating the adsorbed peptide or protein with phenylisothiocyanate (PITC) and subsequently treating the adsorbed protein or peptide with a base;
• c) treating the product of step (b) with 1,4-phenylene diisothiocyanate (DITC);
• d) depositing a polymer onto the adsorbed peptide or protein of step (c), wherein the polymer has functional groups which can be crosslinked;
• and
• e) crosslinking the polymer, to thereby produce a polymer network which entraps the peptide or protein therein, wherein the network does not interfere with subsequent chemical or enzymatic analysis of the protein or peptide.