Xanthene dyes that emit to the red of fluorescein
AssigneeBecton, Dickinson and Company
InventorLinda Lee
Life of this patent
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Abstract
Novel xanthene dyes are disclosed which excite in the range of 450-650 nm and which will emit to the red of fluoroscein. These dyes may be coupled to tagging agents, such as monoclonal antibodies, and used to detect cells in a sample.
Claims21
A method for staining cells in a sample which comprises incubating said with a tagging agent which specifically binds to said cells under conditions sufficient to allow said cells to bind with said tagging agent, said tagging agent being coupled to a fluorescent compound selected from the group consisting of ##STR3## wherein R is CO.sub.2 H or SO.sub.3 H, and X is H or Br when R is CO.sub.2 H and X is H when R is SO.sub.3 H;
- ##STR4##
A method for identifying cells in a sample which comprises:
- (a) incubating said cells with a tagging agent which specifically binds to said cells under conditions sufficient to allow said cells to bind with said tagging agent, said tagging agent being coupled to a fluorescent compound selected from the group consisting of of the formula ##STR7## wherein R is CO.sub.2 H or SO.sub.3 H, and X is H or Br when R is CO.sub.2 H and X is H when R is SO.sub.3 H;
- ##STR8## (b) detecting fluorescence emitted by said bound tagging agent as a measure of the presence of said cells.
A method for discriminating between live cells and dead cells in a sample which comprises:
- (a) incubating said cells with a fluorescent compound selected from the group consisting of ##STR11## wherein R is CO.sub.2 H or SO.sub.3 H, and X is H or Br when R is CO.sub.2 H and X is H when R is SO.sub.3 H;
- ##STR12## under conditions sufficient to allow uptake of said compound by said live cells;
- and
- (b) detecting fluorescence emitted by said compound as a measure of the presence of said live cells.
A method for detecting an esterase activity in a sample of cells comprising
- a) incubating said cells with a fluorescent compound selected from the group consisting of ##STR15## wherein R is CO.sub.2 H or SO.sub.3 H, and X is H or Br when R is CO.sub.2 H and X is H when R is SO.sub.3 H;
- ##STR16## under conditions sufficient to allow uptake of said compound by said cells and cleavage of said compound by said esterase activity
- b) detecting fluorescence emitted by said cleaved compound as a measure of said esterase activity.
Dependencies are inferred from the claim text.