Hemoglobin multiple emulsion
AssigneesIllinois Institute of Technology, Northfield Laboratories, Inc.
InventorsRichard L. Beissinger, Darsh T. Wasan, Lahksman R. Sehgal, Arthur L. Rosen
Life of this patent
Estimating the term…
Abstract
A multiple emulsion of aqueous oxygen carrying material in oil in outer aqueous phase is suitable for provision of oxygen for oxygen transfer processes. A hemoglobin multiple emulsion in physiologically compatible oil in an outer aqueous saline solution is provided in sufficiently small droplet size to provide oxygen flow through blood vessels to desired body tissues or organs thereby providing a blood substitute. A process is provided wherein hemoglobin, a fragile material, is formulated into high hemoglobin content water-in-oil-in-water multiple emulsions while maintaining high yields and high oxygen exchange activity.
Claims35
An oxygen carrying multiple liquid emulsion comprising a primary emulsion comprising aqueous solution of oxygen carrying material in oil selected from the group consisting of mineral oil, vegetable oil and mixtures thereof, emulsified with a primary emulsifier having primary emulsion droplet sizes of up to about 5 microns and a secondary emulsion comprising said primary emulsion in an outer aqueous phase emulsified with a secondary emulsifier having secondary emulsion droplet sizes up to about 10 microns, said multiple emulsion having a viscosity of about 3 to about 9 cp, yield about 85 to about 99 percent, and oxygen carrying capacity about 7 to about 20 volume percent.
An oxygen carrying multiple emulsion according to claim I wherein said multiple emulsion comprises about 10 to about 90 volume percent said primary emulsion, about 10 to about 90 volume percent said outer aqueous phase, and about 0.25 to about 2 weight percent said secondary emulsifier.
A blood substitute comprising a sterile oxygen carrying multiple liquid emulsion comprising a primary emulsion comprising aqueous solution of oxygen carrying material in oil selected from the group consisting of mineral oil, vegetable oil and mixtures thereof, emulsified with a primary emulsifier having primary emulsion droplet sizes of up to about 5 microns and a secondary emulsion comprising said primary emulsion in an outer aqueous phase emulsified with a secondary emulsifier having secondary emulsion droplet sizes up to about 10 microns, said multiple emulsion having a viscosity of about 3 to about 9 cp, yield about 85 to about 99 percent, and oxygen carrying capacity about 7 to about 20 volume percent.
Dependencies are inferred from the claim text.